Journal: PLOS One
Article Title: Activated human B cells produce phospholipase D4-containing extracellular vesicles
doi: 10.1371/journal.pone.0329832
Figure Lengend Snippet: (A) Immunofluorescence microscopy displaying localization of PLD4 (red) at TGN46-tagged trans Golgi networks (green) in resting B cells and translocation of PLD4 from trans Golgi networks in CpG/anti-IgG + M stimulated B cells, compared to the control cells. (B) Immunofluorescence microscopy shows enhanced expression of PLD4 (red) and co-localization of PLD4 and CD63 (green) in CpG/anti-IgG + M stimulated B cells, compared to the control cells. Cell membranes stained with MemBrite appeared thicker in CpG/anti-IgG + M stimulated B cells than in the control cells. (C) Statistical analysis revealing enhanced PLD4 expression in each CpG/anti-IgG + M stimulated B cell compared to the control cells. Bar; 10 μm, **P < 0.01, ***P < 0.001.
Article Snippet: Plasma membranes were stained using the MemBrite ® Fix Cell Surface Staining Kits – MemBrite ® Fix 680/700 (SKU: 30099-T; Biotium, Fremont, CA, USA).
Techniques: Immunofluorescence, Microscopy, Translocation Assay, Control, Expressing, Staining